The MRTC's membership consists of two of the largest hospital networks in Iowa. With organizational "hubs" at Mercy Hospital Medical Center (Des Moines) and North Iowa Mercy Health Center (Mason City), the networks extend to 23 Iowa hospitals, as well as extensive clinic systems and sub-acute care facilities. By virtue of other formal and informal collaborative relationships, the networks reach communities throughout the state. The MRTC provides a vehicle for collaborative efforts among member organizations.

The MRTC is governed by an executive committee consisting of physicians and hospital administrators affiliated with the two hospital networks. Individuals with expertise in specific areas of MRTC interest are routinely recruited to serve on standing and ad hoc committees. Full-time staff include a project director, network services coordinator, technical support coordinator and secretary, as well as site coordinators at the hub hospitals. Part-time site coordinators at other MRTC member organizations to provide a single point of contact for information about telemedicine-related services and activities.

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